FIG. 4.

VAMP3-deficient mice display normal glucose and insulin tolerance. (A) GTT of 16-week-old male mice. d-Glucose (20% solution, 2 g/kg of body weight) was injected intraperitoneally into mice fasted for 12 h. Blood glucose was measured at 0, 30, 60, 90, and 120 min postinjection using a glucometer as described in Materials and Methods. (B) ITT of 16 week-old male mice. Insulin (0.75 U/kg of body weight) was injected intraperitoneally to mice that were fasted for 6 h. Blood glucose was measured immediately before and at 15, 30, and 60 min after injection as described in Materials and Methods. The experimental groups consisted of 10 and 6 mice for GTT and ITT, respectively, and were performed a minimum of three times with similar results.