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. 2021 Jul 30;33(1):e562–e572. doi: 10.1097/CAD.0000000000001180

Fig. 3.

Fig. 3

The cell-penetrating ability of activatable cell-penetrating peptide (ACPP)-p21Ras scFv was detected. (a) ACPP-p21Ras scFv and ACPP-L-p21Ras scFv were found in the A549 cells (brown), while p21Ras scFv was not found in the A549 cells. Immunohistochemical staining, ×40. (b) ACPP-p21Ras scFv and ACPP-L-p21Ras scFv were observed by immunofluorescence assay in A549 cells, while p21Ras scFv was not observed in the cells. Red: Rho-stained of p21Ras scFv, ACPP-p21Ras scFv and ACPP-L-p21Ras scFv, blue: DAPI-stained nuclei (×1000). (c) ACPP-p21Ras scFv and ACPP-L-p21Ras scFv were detected by WB in A549, SW480, Huh7 and U251 cells, but not in Beas-2B cell. DAPI, 4’,6-diamidino-2-phenylindole; WB, western blot.