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. 2021 Jul 30;33(1):e562–e572. doi: 10.1097/CAD.0000000000001180

Fig. 5.

Fig. 5

Activatable cell-penetrating peptide (ACPP)-p21Ras scFv induce apoptosis on A549 cells and H1299 cells in vitro. (a) The cells treated with ACPP-p21Ras scFv, ACPP-L-p21Ras scFv and p21Ras scFv, then were stained with TUNEL kit and then detected by microscope, and the apoptotic cells are indicated with arrows. (b and c) Apoptotic cells are stained with annexin V/propidium iodide, then detected by flow cytometry. Annexin V/propidium iodide assay showed that the apoptotic percentage of A549 cells and H1299 cells treated with ACPP-p21Ras scFv and ACPP-L-p21Ras scFv were higher than that with p21Ras scFv (P < 0.05). TUNEL, terminal deoxynucleotidyl transferase-mediated fluorescein-dUTP nick end labeling.