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HtrA and HtrB levels in the parental strain 168 and midiBacillus. The expression of IsaA was induced with subtilin, and culture samples were collected 2 h after induction. In parallel, samples were collected from noninduced cultures. Cell-associated and extracellular proteins were separated by LDS-PAGE, and the presence of HtrA and HtrB was assessed by Western blotting with polyclonal antibodies specific for HtrA or HtrB.
