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Study design. (A) Representative phase microscopic images of 2D (single cells) and 3D (spheroids) cell constructs; scale bars 100 μm. (B) Schema of study design, experimental groups and outcomes; constructs were cultured in vitro in osteogenic induction medium for 7 days prior to implantation (+7 days). (C) Representative macroscopic images of 8-weeks tissue specimens containing BMSC (a), GPC (b) or cell-free constructs (c); scale bars 2 mm.
