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. 2021 Nov 30;9:694261. doi: 10.3389/fcell.2021.694261

FIGURE 1.

FIGURE 1

Reduced expression of GRK2 characterizes the preeclamptic placenta. (A) GRK2 mRNA levels were determined in the term placenta samples by qRT-PCR. Each dot is representative of a patient sample. (B) Western blotting revealed the protein levels of placental GRK2. Each dot is representative of a patient sample. (C) Immunofluorescence co-staining and quantification of GRK2 (red), CK7 (green; trophoblasts), and DAPI (blue; nuclei) in term placentae. Scale bar, 100 μm. (D) qRT-PCR analysis of GRK2 mRNA abundance in the first-trimester villous explants (n = 8). The tips, branches, and EVT outgrowth cells were isolated along the villus explants on Matrigel-coated wells at 48 h post-explant. Data are shown as the mean ± SEM. PE, preeclampsia; GRK, G Protein-Coupled Receptor Kinase; CTB, cytotrophoblast; STB, syncytiotrophoblast; EVT, extravillous cytotrophoblasts. *P < 0.05, **P < 0.01, ***P < 0.001; ****P < 0.0001.