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. 2021 Nov 30;9:694261. doi: 10.3389/fcell.2021.694261

FIGURE 7.

FIGURE 7

Decreased GRK2 levels or activity undermines migration and invasion in HTR8/SVneo cells, after excluding dead cells. (A) Migration ability of HTR8/SVneo cells assessed by wound closure assay. After 24 h, the wound edges are nearly closed in blank and NC-siRNA groups, while that in GRK2-siRNA group remained apparent distances. While lines, wound edges. Scale bar, 100 μM. (B) Migration ability tests on HTR8/SVneo cells with GSK180736A at different concentrations (0.5, 1, 2.5, and 5 μM) for 24 h. Scale bar, 100 μM. (C) Representative images of invaded HTR8/SVneo cells through chambers’ membrane (100×). The histograms show apparently reduced percentage of invasive cells in GRK2-siRNA group compared to others, which should be interpreted more than necroptosis. (D) Representative images and the analysis of EVTs migration ability tests performed on human villous explants. (E) Representative images of invaded through chambers’ membrane(x100). HTR8/SVneo cells treated with GSK180736A at different concentrations (0.5μM, 1μM, 2.5μM, 5μM) for 24 hrs and invaded through chambers’ membrane(100x). (F) HTR8/SVneo cells administrated with z-VAD-FMK (25μM, 24 hrs) or nec-1 (30 μM, 24 hrs) cannot restore the migration ability of cells. Note: NS, not significant; **P <0.01, ***P <0.001, ****P <0.0001.