Fig. 3.

NAN and IAV are required for mechanical stimuli–induced whole-cell current in lch1 Cho neurons. (A) Current response to a mechanical stimulus (1 μm) was eliminated in nan and iav null mutants but could be rescued by expressing NAN and IAV in lch1 neurons of nan^36a and iav¹ mutants, respectively. Lch1 neurons were clamped at −60 mV (holding potential) in Na⁺/K⁺-based solutions. (B) Bar graph summarizing the peak current (absolute value) of the lch1 neurons in wild-type (WT), nan^36a, nan rescue, iav¹, and iav rescue larvae (n = 10, 7, 8, 9, and 8, respectively; mean ± SEM). (C) The activation time constants of MET currents in larvae with indicated genotype (n = 10, 8, 8, respectively, mean ± SEM). (D) Summary of the adaptation time constants of MET currents in WT, nan rescue, and iav rescue larvae (n = 10, 8, and 8, respectively; mean ± SEM). The unpaired t test was used for comparison between two groups, and one-way ANOVA followed by Holm–Šidák post hoc analysis was used for comparison among multiple groups. ***P < 0.001. NS, not statistically significant. Genotypes are as follows: nan^36a: Iav-Gal4/UAS-GFP; nan^36a/nan^36a. iav¹: iav¹/y; Iav-Gal4/+; UAS-CD8-GFP/+. nan rescue: Iav-Gal4, UAS-GFP/UAS-Nanchung-GFP; nan^36a/nan^36a. iav rescue: iav¹/y; Iav-Gal4, UAS-GFP/+; UAS-Inactive-GFP/+. WT: Iav-Gal4/+; UAS-CD8-GFP/+.