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. 2021 Nov 30;118(49):e2106459118. doi: 10.1073/pnas.2106459118

Fig. 6.

Fig. 6.

The carboxyl-terminal region of IAV contributes to adaptation of MET currents and is essential for the proper localization of NAN-IAV channels. (A and B) Representative traces of single-channel activities in NAN-IAV–, NAN-Δ857-1123-IAV–, NAN-Δ857-990-IAV–, and NAN-Δ991-1123-IAV–expressing S2 cells with 2 μM NAM in internal solution. S2 cells were recorded at −60 mV (holding potential) in Na+/K+-based solutions. (C) Single-channel current amplitudes (absolute value) of wild-type and truncated NAN-IAV at −60 mV (holding potential) in Na+/K+-based solutions (n = 10, 6, 7, and 5; NS, not statistically significant). One-way ANOVA followed by Holm–Šidák post hoc analysis was used for comparison among multiple groups. (D) Wild-type and truncated IAV expression in Cho neurons of iav1 larvae. (Scale bar, 20 μm.) GFP signals are enhanced with an anti-GFP antibody (black). (E) Representative traces of MET current from wild-type and Δ857-990-iav larvae; lch1 neurons were held at −60 mV in Na+/K+-based solutions. Cyan lines represent single exponential fits of the adaptation of MET currents; purple lines represent single exponential fits of the activation of MET currents. (F) Deletion of 857-990 residues of IAV did not alter the activation time constant of MET currents in lch1 neurons (mean ± SEM, n = 10, 9; NS, not statisticlly significant, unpaired t test). (G) Deletion of 857-990 residues of IAV prolonged the adaptation time constant of MET currents (mean ± SEM, n = 10 and 9; ***P < 0.001, unpaired t test). (H) Summary of MET current amplitudes (absolute value) in lch1 neurons of wild-type and Δ857-990-iav larvae (mean ± SEM, n = 10 and 9; NS, not statistically significant, unpaired t test). Genotypes are as follows: for D, iav-GFP: iav1/y; Iav-Gal4/+; UAS-iav-GFP/+. Δ857-1123-iav-GFP: iav1/y; Iav-Gal4/+; UAS-Δ857-1123-iav-GFP/+. Δ857-990-iav-GFP: iav1/y; Iav-Gal4/+; UAS-Δ857-990-iav-GFP/+. Δ991-1123-iav-GFP: iav1/y; Iav-Gal4/+; UAS-Δ991–1123-iav-GFP/+. For E-H, Δ857-990-iav: iav1/y; Iav-Gal4/+; UAS-Δ857-990-iav-GFP/+. WT: Iav-Gal4/+; UAS-CD8-GFP/+.