FIG. 5.

DNase I footprinting analysis of 3′ deletions bound to immobilized TFIID. DNA fragments corresponding to the wild-type promoter and a series of 3′ deletions were end labeled on the nontranscribed strand near −194. The other end of each fragment was established by a restriction enzyme cut in the vector located well beyond the region contacted by TFIID. Samples in lanes 3, 7, 11, 15, 19, and 23 are DNA isolated from TFIID after DNase I treatment. The lanes flanking each side of the TFIID-bound samples were derived by DNase I treatment of unbound DNA. Lanes 1, 5, 9, 13, 17, and 21 correspond to purine-cutting patterns for each of the respective promoter fragments.