Cumulative adult fly output at different temperatures: (A) 23 °C, (B) 25 °C, (C) 27 °C, (D) 20 °C. Shown is the cumulative adult progeny output of four replicates in which five females were crossed to five males over 12 d. Crosses were heterozygous ♀TS-ClvRdbe/+ × ♂w1118 in magenta, homozygous ♀TS-ClvRdbe/TS-ClvRdbe × ♂TS-ClvRdbe/TS-ClvRdbe in yellow, ♀w1118 × ♂TS-ClvRdbe/TS-ClvRdbe in violet, ♀w1118 × ♂w1118 (control) in blue, and the original non-TS ♀ClvRdbe × ♂ClvRdbe (control) in green.