Figure 5.

Ct stimulates Th17 cells rather than ILC3s in the ileum. Flow cytometric analyses were conducted on 8-colour FACS Verse and three panels were used to analyzing the intestinal immune cells. The single cells were first gated on SSC-A vs FSC-A and FSC-A vs FSC-H, and then CD45⁺FVS780^- cells (CD45⁺Live⁺ cells) were gated out. CD3e⁺RORγt⁺ cells were gated out from CD45⁺CD3e^- cells. CD45⁺CD3e^- cells were gated out from CD45⁺FVS780^- cells and CD127⁺RORγt⁺ cells were gated out from CD45⁺CD3e^- cells. (A) Proportions of Th17 cells (CD45⁺CD3e⁺RORγt⁺ cells). (B) Proportions of ILC3s (CD45⁺CD3e^-CD127⁺RORγt⁺). Each point presented a mouse. Data were presented as mean ± SEM. The significant difference was analyzed by two-way unpaired t-tests. *P < 0.05, **P < 0.01.