Fig. 2.

Effect of age on caveolin-1 expression. Expression of caveolin-1 in total (A), membrane (B), and cytosolic (C) fractions of lysate from rat bladder tissue in which the mucosa was removed. β-Actin expression (43 kDa) was used as control. Lane 1 = young; lane 2 = 6 months; lane 3 = 12 months. Corresponding graphs below show relative expression of caveolin-1 (normalized to β-actin) in total, membrane, and cytosolic fractions in the three age groups (n = 3). Relative expression of α and β isoforms (D) of caveolin-1 (normalized to β-actin) in the membrane lysate of bladder tissue was not different among the three age groups (n = 4 for young and 3 for the other age groups). E: Quantification of Western blot showing no change in the expression of cavin-1 in total lysate from each age group (n = 3). β-Actin was used as loading control. Bladders used for extraction of cytosolic and membrane fractions were procured from different animals than those used for extraction of total protein. Y, young; 6M, 6 months; 12M, 12 months.