Effect of age on expression of caveolin-2 in rat bladder. A: Western blot for caveolin-2 (α, β, γ isoforms) in total protein lysate of bladders procured from young, 6 months, and 12-month-old animals (n = 3 for each group) from which the mucosa was removed. β-Actin (43 kDa) was used as control. B: Corresponding graph depicts the quantitative changes in caveolin-2 expression with age, normalized by β-actin. Y, young (n = 3); 6M, 6 months (n = 3); 12M, 12 months (n = 3). *Significant compared to young (P < 0.05).