Table 1. Oligonucleotide primers used for one step PCR to detect S mutations.
| Primer | Type | Start | End | Sequences (5′-3′) | Tm (°C) | Size (bp) |
|---|---|---|---|---|---|---|
| 69S | F. primer | 21672 | 21696 | TGTAAAACGACGGCCAGTATTACCCTGACAAAGTTTTCAGATC | 65.8 | 294 |
| R. primer | 21907 | 21930 | CAGGAAACAGCTATGACGCGTTATTAACAATAAGTAGGGAC | 62.8 | ||
| 144S | F. primer | 21843 | 21869 | TGTAAAACGACGGCCAGTCCACTGAGAAGTYTAACATAATAAGAG | 63.8 | 513 |
| R. primer | 22295 | 22320 | CAGGAAACAGCTATGACTCACCAGGAGTCAAATAACTTCTAT | 64.1 | ||
| 417S | F. primer | 22226 | 22249 | TGTAAAACGACGGCCAGTGCTTTAGAACCATTGGTAGATTTG | 65.2 | 759 |
| R. primer | 22923 | 22949 | CAGGAAACAGCTATGACGTTTGAGATTAGACTTCCTAAACAATC | 65.1 | ||
| 484S | F. primer | 22874 | 22898 | TGTAAAACGACGGCCAGTTCTAAYAAICTTGATTCTAAGGTTG | 62.8 | 375 |
| R. primer | 23192 | 23213 | CAGGAAACAGCTATGACCKCCTGTGCCTGTTAAACCATT | 66.3 | ||
| 570S | F. primer | 23108 | 23130 | TGTAAAACGACGGCCAGTGAACTTCTACATGCACCAGCAAC | 66.2 | 739 |
| R. primer | 23790 | 23811 | CAGGAAACAGCTATGACCTGCATTCAGTTGAATCACCAC | 64.5 |
Primers for specific target mutations in Wuhan-Hu-1-CoV were designed and conserved regions of the S gene are highlighted in bold. Primers were extended by tagging the 5′ side with M13 as a universal sequencing primer. Abbreviations: Tm, melting temperature; F. primer, forward primer; R. primer, reverse primer; Δ, deletion; Y, C or T; K, G or T; I, inosine.