Table 2. PCR results and lineage information associated with the respiratory pathogens used in this study.
| Group | Strain | Source | Lineage | CDC classification | PCR result |
|---|---|---|---|---|---|
| Virus | SARS-CoV-2 | Twistbio-601443 | alpha (B.1.1.7) | VOC | Positive |
| SARS-CoV-2 | Twistbio-678597 | beta (B.1.351) | VOC | Positive | |
| SARS-CoV-2 | Twistbio-710528 | alpha (B.1.1.7) | VOC | Positive | |
| SARS-CoV-2 | Twistbio-79683 | gamma (P.1) | VOC | Positive | |
| SARS-CoV-2 | NCCP-43381 | alpha (B.1.1.7) | VOC | Positive | |
| SARS-CoV-2 | NCCP-43382 | beta (B.1.351) | VOC | Positive | |
| SARS-CoV-2 | NCCP-43390 | delta (B.1.617.2) | VOC | Positive | |
| SARS-CoV-2 | NCCP-43384 | epsilon (B.1.427) | VOI | Positive | |
| SARS-CoV-2 | NCCP-43385 | epsilon (B.1.429) | VOI | Positive | |
| SARS-CoV-2 | NCCP-43386 | eta (B.1.525) | VOI | Positive | |
| SARS-CoV-2 | NCCP-43387 | iota (B.1.526) | VOI | Positive | |
| SARS-CoV-2 | NCCP-43389 | kappa (B.1.617.1) | VOI | Positive | |
| SARS-CoV-2 | NCCP-43383 | zeta (P.2) | Not classified | Positive | |
| SARS-CoV-2 | NCCP-43330 | Not provided | Not classified | Positive | |
| SARS-CoV-2 | NCCP-43331 | Not provided | Not classified | Positive | |
| SARS-CoV-2 | NCCP-43342 | Not provided | Not classified | Positive | |
| SARS-CoV-2 | NCCP-43343 | Not provided | Not classified | Positive | |
| SARS-CoV-2 | NCCP-43344 | Not provided | Not classified | Positive | |
| SARS-CoV-2 | NCCP-43345 | Not provided | Not classified | Positive | |
| SARS-CoV-2 | Zeptometrix-0810587CFHI | Not provided | Not classified | Positive | |
| SARS-CoV-2 | Zeptometrix-0810589CFHI | Not provided | Not classified | Positive | |
| SARS-CoV-2 | Zeptometrix-0810590CFHI | Not provided | Not classified | Positive | |
| Coronavirus OC43 | ATCC VR1558 | Not provided | Not classified | Negative | |
| Coronavirus 229E | ATCC-VR 740 | Not provided | Not classified | Negative | |
| Influenza A virus | ATCC VR-810 | Not provided | Not classified | Negative | |
| Influenza B virus | ATCC VR-1735 | Not provided | Not classified | Negative | |
| Influenza A H1N1 | ATCC VR-1683 | Not provided | Not classified | Negative | |
| Influenza A H3N2 | ATCC VR-822 | Not provided | Not classified | Negative | |
| Influenza A H1N1 | ATCC VR-219 | Not provided | Not classified | Negative | |
| Influenza A H3N2 | ATCC VR-547 | Not provided | Not classified | Negative | |
| Respiratory syncytial virus A | ATCC VR-26 | Not provided | Not classified | Negative | |
| Respiratory syncytial virus B | ATCC VR-955 | Not provided | Not classified | Negative | |
| Parainfluenza type 1 | ATCC VR-1380 | Not provided | Not classified | Negative | |
| Bacteria | Staphylococcus aureus | ATCC-29213 | Not provided | Not classified | Negative |
| Streptococcus pneumoniae | ATCC-49619 | Not provided | Not classified | Negative | |
| Streptococcus pyogenes | ATCC-19615 | Not provided | Not classified | Negative | |
| Pseudomonas aeruginosa | ATCC-27853 | Not provided | Not classified | Negative | |
| Enterobacter aerogenes | ATCC-13048 | Not provided | Not classified | Negative | |
| Enterobacter cloacae | ATCC-13047 | Not provided | Not classified | Negative | |
| Corynebacterium spp. | ATCC-51860 | Not provided | Not classified | Negative | |
| Moraxella catarrhalis | KCCM-42706 | Not provided | Not classified | Negative | |
| Haemophilus influenzae | ATCC-9007 | Not provided | Not classified | Negative | |
| Fungi | Aspergillus fumigatus | Zeptometrix-Z014 | Not provided | Not classified | Negative |
| Aspergillus flavus | Zeptometrix-Z013 | Not provided | Not classified | Negative | |
| Aspergillus niger | Zeptometrix-Z105 | Not provided | Not classified | Negative | |
| Aspergillus terreus | Zeptometrix-Z016 | Not provided | Not classified | Negative | |
| Aspergillus nidulans | ATCC-38163 | Not provided | Not classified | Negative | |
| Aspergillus versicolor | ATCC-11730 | Not provided | Not classified | Negative |
Strains selected for assay validation (22 strains of SARS-CoV-2, 11 strains of other virus, 9 strains of bacteria, and 6 strains of fungi). Strain information, provided by the company from which the strain was acquired, is shown. Abbreviations: SARS-CoV-2, Severe acute respiratory syndrome-related coronavirus 2; Twistbio, Twist Bioscience; NCCP, National Culture Collection for Pathogens; ATCC, American Type Culture Collection; KCCM; Korean Culture Center of Microorganisms; VOC, variants of concern; VOI, variants of interest; CDC, Centers for Disease Control and Prevention.