Table 3. Evaluation of detection limit in target regions.
| Primer pair | Conc. (TCID50/mL) | Reactions | Positive | Positive rate (%) | LOD 95% level (TCID50/mL) |
|---|---|---|---|---|---|
| 69S | 1.4 × 103 | 25 | 25 | 100 | 3.7 × 101 |
| 1.4 × 102 | 25 | 25 | 100 | ||
| 1.4 × 101 | 25 | 20 | 80 | ||
| 1.4 × 100 | 25 | 7 | 28 | ||
| 1.4 × 10−1 | 25 | 0 | 0 | ||
| 1.4 × 10−2 | 25 | 0 | 0 | ||
| 144S | 1.4 × 103 | 25 | 25 | 100 | 9.8 × 101 |
| 1.4 × 102 | 25 | 25 | 100 | ||
| 1.4 × 101 | 25 | 4 | 16 | ||
| 1.4 × 100 | 25 | 0 | 0 | ||
| 1.4 × 10−1 | 25 | 0 | 0 | ||
| 1.4 × 10−2 | 25 | 0 | 0 | ||
| 417S | 1.4 × 103 | 25 | 25 | 100 | 6.6 × 101 |
| 1.4 × 102 | 25 | 25 | 100 | ||
| 1.4 × 101 | 25 | 8 | 32 | ||
| 1.4 × 100 | 25 | 0 | 0 | ||
| 1.4 × 10−1 | 25 | 0 | 0 | ||
| 1.4 × 10−2 | 25 | 0 | 0 | ||
| 484S | 1.4 × 103 | 25 | 25 | 100 | 3.9 × 101 |
| 1.4 × 102 | 25 | 25 | 100 | ||
| 1.4 × 101 | 25 | 18 | 72 | ||
| 1.4 × 100 | 25 | 1 | 4 | ||
| 1.4 × 10−1 | 25 | 0 | 0 | ||
| 1.4 × 10−2 | 25 | 0 | 0 | ||
| 570S | 1.4 × 103 | 25 | 25 | 100 | 7.2 × 101 |
| 1.4 × 102 | 25 | 25 | 100 | ||
| 1.4 × 101 | 25 | 7 | 28 | ||
| 1.4 × 100 | 25 | 0 | 0 | ||
| 1.4 × 10−1 | 25 | 0 | 0 | ||
| 1.4 × 10−2 | 25 | 0 | 0 |
The 5-SSG primer-PCR reactions performed using ten-fold diluted positive samples. The LOD 95% data were estimated using the probit regression analysis. Abbreviations: Conc., concentration; TCID50, median tissue culture infective dose; LOD, limit of detection.