Table 1. Test results for SARS-CoV-2 in nasopharyngeal and semen samples of the 32 enrolled patients.
| Patient | Time between onset of symptoms and nasopharyngeal swab (days) | Time between nasopharyngeal samples and semen (days) | SARS-CoV-2 detection in the nasopharyngeal swab | RT-PCR system | Cycle thresholds (Ct) in the nasopharyngeal swab | SARS-CoV-2 detection | ||
|---|---|---|---|---|---|---|---|---|
| Semen | Seminal plasma | Spermatozoa pellet | ||||||
| 1 | 5 | 1 | Positive | ELITe InGenius | RdRp gene = 18.2; N gene = 19.5; E gene = 16.8 | Negative | Negative | Negative |
| 2 | 5 | 1 | Positive | ELITe InGenius | RdRp gene = 23.0; N gene = 24.6; E gene = 22.2 | Positive | Positive | Negative |
| 3 | 7 | 0 | Positive | Altona | E gene = 33.6 | Negative | Negative | Negative |
| 4 | 7 | 0 | Positive | Altona | E gene = 18.0 | Negative | Negative | Negative |
| 5 | 3 | 1 | Positive | Altona | E gene = 18.4 | Negative | Negative | Negative |
| 6 | 2 | 0 | Positive | Altona | E gene = 18.4 | Negative | Negative | Negative |
| 7 | 6 | 1 | Positive | Altona | E gene = 26.9 | Negative | Negative | Negative |
| 8 | 5 | 0 | Positive | Altona | E gene = 16.0 | Negative | Negative | Negative |
| 9 | 3 | 1 | Positive | Altona | E gene = 16.3 | Negative | Negative | Negative |
| 10 | 4 | 0 | Positive | Altona | E gene = 16.0 | Negative | Negative | Negative |
| 11 | 3 | 1 | Positive | Altona | E gene = 18.0 | NDb | Negative | NDb |
| 12 | 5 | 1 | Positive | ELITe InGenius | RdRp gene = 18.9; N gene = 19.0; E gene = 19.2 | Negative | Negative | Negative |
| 13 | 5 | 1 | Positive | ELITe InGenius | RdRp gene = 14.4; N gene = 19.0; E gene = 16.6 | Negative | Negative | Negative |
| 14 | 5 | 1 | Positive | Altona | E gene = 22.7 | Uninterpretable (Inhibitors) | Negative | Negative |
| 15 | 1 | 1 | Positive | Altona | E gene = 20.4 | Negative | Negative | Negative |
| 16 | 0 | 0 | Positive | Altona | E gene = 26.1 | Negative | Negative | Negative |
| 17 | 3 | 0 | Positive | Altona | E gene = 24.6 | Negative | Negative | Negative |
| 18 | 6 | 0 | Positive | Altona | E gene = 25.0 | Negative | Negative | Uninterpretable (Inhibitors) |
| 19 | 2 | 0 | Positivea | Altona | E gene = 27.5 | Negative | Negative | Negative |
| 20 | 2 | 1 | Positive | Altona | E gene = 33.3 | Negative | Negative | Negative |
| 21 | 4 | 0 | Positivea | Altona | E gene = 17.8 | Negative | Negative | Negative |
| 22 | 2 | 0 | Positivea | Altona | E gene = 17.6 | Negative | Negative | Negative |
| 23 | 8 | 0 | Positivea | Altona | E gene = 27.5 | Negative | Negative | Negative |
| 24 | 1 | 0 | Positivea | CFX96 | N1 gene = 25; N2 gene = 25 | Negative | Negative | Negative |
| 25 | 3 | 1 | Positivea | Altona | E gene = 30.4 | Uninterpretable (Inhibitors) | Uninterpretable (Inhibitors) | Negative |
| 26 | 1 | 1 | Positive | Altona | E gene = 35.9 | Negative | Negative | Negative |
| 27 | 6 | 1 | Positivea | Altona | E gene = 32.7 | Negative | Negative | NDb |
| 28 | 1 | 0 | Positivea | ABI PRISM 7500 | N gene = 15.0 | Negative | Negative | Negative |
| 29 | 4 | 1 | Positivea | Altona | E gene = 20.6 | Negative | Negative | Negative |
| 30 | 4 | 0 | Positivea | Altona | E gene = 21.3 | Negative | Negative | Negative |
| 31 | 1 | 0 | Positive | CFX96 | N1 gene = 18; N2 gene = 19 | Negative | Negative | Negative |
| 32 | 7 | 0 | Positive | CFX96 | N1 gene = 31; N2 gene = 32 | Negative | Negative | Negative |
a Presence of mutations suggestive of the B1.1.7 (N501Y.V1—UK variant)
b ND: Not done
The time between onset of symptoms and semen collection (days) and the time between semen and nasopharyngeal samples (days) are presented for each of the 32 enrolled COVID-19 patients. The cycle threshold (Ct) in the nasopharyngeal swabs and the SARS-CoV-2 detection in semen samples are described.