Table 1. Interactive effects of 2-way ANOVA for microbial load, immune signaling pathways and AMPs (qPCR Type III Fixed effects).

Arrows indicate up or down-regulation of gene expression during fungal or Wolbachia infection.

	Target	Effect	Ae. albopictus			Cx. pipiens
			Num/Den DF	F Value	Pr>F	Num/Den DF	F Value	Pr>F
Imm. Sign. Pathways	PGRP-LC	F	2/54	↑7.57	0.0013	2/57	↑45.38	<0.0001
		W	1/54	0.77	0.3831	1/57	1.21	0.2763
		F*W	2/54	1.96	0.1509	2/57	0.38	0.6844
	PGRP-S1	F	2/47	↑23.56	<0.0001
		W	1/47	2.31	0.1355
		F*W	2/47	2.31	0.1109
	Rel1	F	2/54	↑18.72	<0.0001	2/57	1.94	0.1525
		W	1/54	0.01	0.9078	1/57	0.31	0.5782
		F*W	2/54	1.36	0.2662	2/57	0.07	0.936
	Rel2	F	2/51	↑12.33	<0.0001	2/55	↑4.62	0.014
		W	1/51	0.03	0.8641	1/55	0.05	0.8214
		F*W	2/51	0.04	0.9565	2/55	0.47	0.6293
Antimicrobial Effectors	CecA	F	2/54	↑36.64	<0.0001	2/55	↑9.2	0.0004
		W	1/54	2.47	0.1222	1/55	↓18.96	<0.0001
		F*W	2/54	0.55	0.5782	2/55	3.04	0.0557
	DefC	F	2/52	↑55.37	0.0001	2/50	↑16.84	<0.0001
		W	1/52	0.99	0.3248	1/50	0.66	0.4199
		F*W	2/52	1.33	0.2735	2/50	4.68	0.0137
	Lys	F	2/53	↑43.29	<0.0001	2/55	↑5.15	0.0089
		W	1/53	0.23	0.6354	1/55	0.4	0.5275
		F*W	2/53	0.38	0.6862	2/55	1.9	0.1588
	Tep22	F	2/52	0.59	0.5594	2/56	↑7.32	0.0015
		W	1/52	↑12.16	0.001	1/56	↓4.74	0.0337
		F*W	2/52	3.73	0.0305	2/56	0.22	0.806
Oxidative Stress	Duox	F	2/53	↑4.21	0.02	2/59	0.03	0.973
		W	1/53	2.59	0.1135	1/59	0.47	0.4955
		F*W	2/53	2.49	0.0922	2/59	0.33	0.7234
	DuoxA	F	2/55	↑3.39	0.0408
		W	1/55	1.31	0.2565
		F*W	2/55	0.05	0.9471
Antioxidant Defense	Catalase	F	2/51	0.86	0.4283	2/56	↑6.56	0.0028
		W	1/51	↓8.29	0.0058	1/56	↑15.46	0.0002
		F*W	2/51	4.84	0.0119	2/56	3.82	0.0278
	GPX	F	2/55	↑10.21	0.0002	2/55	0.28	0.758
		W	1/55	0.18	0.6705	1/55	0.5	0.4816
		F*W	2/55	0.41	0.6662	2/55	1.47	0.2389