KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
|
| ||
| Anti-LIN28B (D4H1) | Cell Signaling | 11965; RRID: AB_2750978 |
| Anti-LIN28A (6D1F9) | Cell Signaling | 5930; RRID: AB_1903976 |
| Anti-V5 | MBL | M167-3; RRID: AB_1953024 |
|
| ||
| Chemicals, peptides, and recombinant proteins | ||
|
| ||
| Doxycycline hydrochloride | Sigma | D3447 |
| LIN28-Tat | Peprotech | 110-06 |
|
| ||
| Critical commercial assays | ||
|
| ||
| Illumina Stranded mRNA Prep | Illumina | 20040532 |
| miScript Primer Assays | QIAGEN | 218300 |
|
| ||
| Deposited data | ||
|
| ||
| RNA sequencing data for Polysome Fractionation | Tan et al. (2019) | GEO: GSE109423 |
| RNA sequencing data | This Manuscript | GEO: GSE178259 |
| Uncropped Western Blots | Mendeley | https://data.mendeley.com/datasets/g5sznxfzfw/1 |
|
| ||
| Experimental models: Cell lines | ||
|
| ||
| Human HEK293A | This Manuscript | N/A |
| LIN28B knockout (human HEK293A) | Tan et al. (2019) | N/A |
| Dicer Knockout mouse embryonic stem cells | Dr. Anthony Leung (JHU) | N/A |
| V6.5 mouse embryonic stem cells | This Manuscript | N/A |
|
| ||
| Recombinant DNA | ||
|
| ||
| LIN28B transgene PiggyBAC | This Manuscript | N/A |
| LIN28B sponge transgene PiggyBAC | This Manuscript | N/A |
|
| ||
| Software and algorithms | ||
|
| ||
| Random Forest Classifiers | Breiman (2001) | https://www.stat.berkeley.edu/~breiman/RandomForests/ |