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. Author manuscript; available in PMC: 2022 Mar 20.
Published in final edited form as: Nat Cancer. 2021 Sep 20;2(9):919–931. doi: 10.1038/s43018-021-00245-1

Extended Data Fig. 4. 4Gy-RT elicited neither extensive tumor cell death nor increased cross-presenting DCs in bronchial lymph nodes.

Extended Data Fig. 4

a. Representative tiled immunofluorescent images of radiated HKP-1 lungs showing the impact of various doses of RT on tumor cell death. Cleaved caspase-3 (white) and E-cadherin+ tumor islets (red) were stained. Scale Bar: 100μM. Right panel: quantification of total fluorescence arear of cleaved caspase3. (n = 6 lung sections from 3 mice/treatment. Data are mean ± SEM. ns: non-significant, **** P ≤ 0.0001. One-way ANOVA with Sidak’s post-hoc test.

b. Representative flow-cytometric plots showing the impact of 4Gy-RT on CD103+ DCs in bronchial lymph nodes (BrLNs). BrLNs were collected, processed to single cell suspension, and stained with antibodies as indicated. Flow events were gated as cells, single cells, Epcam−/CD3−, CD8a+, and MHCII+. Flow contour plots were representative of 2 independent experiments. Data are pooled sample from 3 mice/treatment.