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. Author manuscript; available in PMC: 2022 Mar 20.
Published in final edited form as: Nat Cancer. 2021 Sep 20;2(9):919–931. doi: 10.1038/s43018-021-00245-1

Extended Data Fig. 6. 4Gy-RT activates club cells to mediate immune activation.

Extended Data Fig. 6

a, Flow cytometry analysis of club cells (CD45−/Epcamhi/CC10+) in naive lungs after different doses of RT. Flow events were gated sequentially as cells and single cells first as described in Extended data Fig.1b. Representative of n = 5 mice. b, Club cell counts/100,000 single cell events. n=5 mice, ****P< 0.0001, one-way ANOVA with Tukey’s post-hoc test. c, Representative histograms showing the increased proliferation rate (Ki67+) of club cells after 4Gy-RT.

Representative of n = 5 mice. d. Cytokine production by T cells in response to 4 Gy-RT relied on club cells. Representative flow cytometry plots showing the IFNγ+/TNFα+/CD4+ T cells and GzmB+/CD8+ T cells upon 4Gy-RT in HKP1-bearing lungs of mice that were pharmacologically (naphthalene) or genetically (DT) depleted of club cells. Representative of n = 4 mice. 0Gy- and vehicle-treated mice served as controls.