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. Author manuscript; available in PMC: 2022 Mar 20.
Published in final edited form as: Nat Cancer. 2021 Sep 20;2(9):919–931. doi: 10.1038/s43018-021-00245-1

Figure 7. Club secretory proteins reduce immunosuppressive functions of MDSCs.

Figure 7.

a. Western blot analyses of Jak-Stat and Ras/MAPK signaling in MDSCs in response to club secretory proteins. BM-derived MDSCs were treated with GM-CSF/IL-6 (positive control), PBS (negative control), HKP1 supernatant with and without Club cocktail, or BALF collected from 0Gy- or 4Gy-RT-treated HKP1 lungs. a representative blot of n = 2 independent repeats with similar results.

b. RT-PCR analyses of MDSC immunosuppressive and inflammatory mediators. MDSCs were treated as described in a. n=3 independent experiments. Data are mean ± SEM. one-way ANOVA with Sidak’s post-hoc test. For 0Gy vs. 4Gy-RT comparison: **P=0.0055 (Il1b); ***P < 0.0001 (Ptgs2); ***P=0.0008 (Tnf); ***P=0.0004 (Cd274); P=0.47 (Arg1); ***P=0.0002 (Arg2); ***P=0.0007 (Nos2); P=0.44 (Il6). For cont vs. club cocktail comparison: ***P=0.0003 (Il1b); **P= 0.0026 (Ptgs2); **P= 0.0045 (Tnf); *P= 0.031 (Cd274); ***P=0.0002 (Arg1); ***P=0.0008 (Arg2); **P=0.0014 (Nos2); ***P=0.0007 (Nos2); **P=0.0069 (Il6).