FIG. 2.

LPS induction of AP mRNAs as measured by slot blot analysis on STAT3 conditional-mutant (open bars) and control (solid bars) mice. The mice were injected with either LPS or saline solution 4 days after poly(I · C) treatment, and their livers were collected after 0, 1.5, 6, 12, and 24 h, as indicated. Total RNA was extracted, denatured, transferred to nylon membranes by slot blotting, and hybridized with the indicated cDNA probes. The results, shown as mean values + standard errors of the mean of at least five mice per group, were plotted after normalization with GAPDH as an internal control. All values obtained after saline injection were uniform and were therefore pooled and shown collectively as time zero, representing the steady-state value.