FIG. 6.
EMSAs and anti-MAPK detection with liver extracts from STAT3 conditional-mutant and control mice. The mice were treated as described in the legend to Fig. 2. (A) Livers were collected 4.5 and 9 h after LPS injection, and nuclear extracts were prepared immediately. Total nuclear extracts were incubated with 32P-labeled double-stranded oligonucletides carrying binding sites for NF-κB (from the human immunodeficiency virus 3′ long terminal repeat) or STAT3-STAT1 (corresponding to the sis-inducible element on the c-fos promoter). (B) To detect MAPK phosphorylation, total extracts from mice treated with LPS for the indicated lengths of time were subjected to Western blot analysis with anti-phospho-(p44/42) MAPK antibodies (P-MAPK). The blots were stripped and reprobed with anti-MAPK antibodies (MAPK). +, positive; −, negative.