Figure 4. Regulation of inflammation-resolution responses in hGPR32_mycTg×Fpr2^–/–×Apoe^–/– mice during acute peritonitis.

Fpr2^–/–×Apoe^–/– (white box) and hGPR32_mycTg×Fpr2^–/–×Apoe^–/– (gray box) mice were subjected to a zymosan challenge (1 mg/mouse, i.p.), and peritoneal exudates were collected at the indicated time points. (A) Exudate neutrophil numbers (Ly6G⁺ cells) and (B) cytokine levels after 4 hours (n = 7, white box;n = 3, gray box). (C) Exudate macrophage/neutrophil ratio after 24 hours (n = 17, white box; n =11, gray box) and 48 hours (n = 8, white box; n =7, gray box), respectively.(D) Efferocytosis assessed as Ly6G⁺ macrophages and (E) Alox15 mRNA expression in exudate cells after 24 hours (n = 11, white box; n =5, gray box) and 48 hours (n = 6, white box; n =4, gray box), respectively. *P < 0.05; **P < 0.01 between genotypes. (F) Total exudate cells and (G) neutrophils in Fpr2^–/–×Apoe^–/– (open) and hGPR32_mycTg×Fpr2^–/–×Apoe^–/– (filled) mice injected with vehicle (black outline) or AT-RvD1 (100 ng/mouse; blue outline) immediately prior to zymosan challenge (vehicle: n = 10 for Fpr2^–/–×Apoe^–/– and n = 7 for hGPR32_mycTg×Fpr2^–/–×Apoe^–/–; AT-RvD1, n = 8 for both genotypes). *P < 0.05; **P < 0.01 vs. vehicle. Results are expressed as median with minimum to maximum bars. Unpaired Student’s t test was used to compare 2 groups, and 2-way ANOVA was used for comparing multiple groups.