Figure 7. BACH2 immunostaining in human pancreata.

(A and B) Coimmunostaining of ACH2 (green), insulin (white), glucagon (red), and DAPI (blue) in human ND (A) or T2D pancreata (B). INS⁺GCG⁺ bihormonal cells expressing BACH2 are indicated by the pink arrows. Orange arrows indicate BACH2-positive β cells and white arrows indicate BACH2-positive α cells. Scale bars: 20 μm. (C–E) Ratio of BACH2-positive cells to INS⁺GCG^– β cells (C), GCG⁺INS^– α cells (D), and INS⁺GCG⁺ bihormonal cells (E). All data are expressed as mean ± SEM. **P < 0.01, ***P < 0.001 by 2-tailed, unpaired Student’s t test performed between the 2 groups (n = 9 per group).