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. 2021 Dec 15;131(24):e147031. doi: 10.1172/JCI147031

Figure 2. CircEsyt2 modulates vascular remodeling in vivo.

Figure 2

(A) Schema of the generation of the vascular remodeling model in C57BL/6J mice. Carotid arteries were collected 28 days after wire injury. (B) qRT-PCR to check for the expression of circEsyt2 in carotid arteries. *P < 0.05 vs. sham. n = 4. (C) Schema of the generation of VSMC lineage tracing mice (Tagln-CreERT2/tdTomato) (left) and vascular remodeling model (right). Tamoxifen was injected intraperitoneally 28 days prior to the wire injury of carotid arteries. (D) FISH assay of carotid arteries treated in C. Left: design of FISH probe against circEsyt2. Right: representative fluorescence images of DAPI (blue), circEsyt2 (green) and tdTomato (red). The sham group served as control. Scale bars: 50 μm. Lu, lumen. Data are mean ± SEM. Two-sided unpaired t test for B.