Skip to main content
. 2021 Dec 15;131(24):e147031. doi: 10.1172/JCI147031

Figure 9. CircEsyt2 regulates PCBP1-dependent p53β splicing.

Figure 9

(A) Predicted interaction of human PCBP1 and pre-mRNA p53 by catRAPID. The binding sites are depicted in red. (B) RIP-qPCR confirming the direct binding of PCBP1 and pre-mRNA p53 in HASMCs. ***P < 0.001 vs. IgG. n = 3. (C) RIP-qPCR to check for the expression of pre-mRNA p53 pulled down by the U2AF65 antibody in HASMCs with the knockdown of circEsyt2 (si-circ), PCBP1 (si-PCBP1), or both. *P < 0.05, **P < 0.01 vs. scr. n = 3. (D) RIP-qPCR to check for the expression of pre-mRNA p53 pulled-down by PCBP1 antibody in circEsyt2-silenced HASMCs. *P < 0.05 vs. scr. n = 3. (E) Western blotting (top) and qRT-PCR (bottom) to check for the expression of p53 and p53β in PCBP1-silenced HASMCs. **P < 0.01 vs. scr. n = 3. (F) Western blotting (top) and qRT-PCR (bottom) to check for the expression of p53β following the silencing of circEsyt2 (si-circ) only or with PCBP1 (si-circ+si-PCBP1). *P < 0.05 vs. scr. n = 3. Data are mean ± SEM. Two-sided unpaired t test for B, C, and E. One-way ANOVA test with Dunnett’s T3 post hoc test for C and F.