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. 2021 Dec 15;131(24):e145501. doi: 10.1172/JCI145501

Figure 1. CD45-SAP and cKit-SAP are similarly effective as conditioning agents for syngeneic HSCT.

Figure 1

(A) Inhibition of B6 bone marrow colony formation in vitro by ADCs or control conjugates. Mean colony counts (from duplicate plates) from 1 representative of 3 experiments are shown. (B) In vivo depletion of bone marrow CD150+CD48– LSK cells (HSC) and CFUs 7 days after infusion with the indicated conjugates. Mice were pooled from 2 to 4 experiments; the same cohort of untreated mice was used to compare with the CD45-SAP and cKit-SAP treatment groups. (C and D) Schematic and results for syngeneic HSCT in mice conditioned with the indicated conjugates. Donor chimerism overall and by lineage (C) and CBCs (D) are displayed and were pooled from 2 to 3 experiments. (E) Secondary HSCT using whole marrow from B6-GFP→B6 primary recipients that were conditioned with the indicated ADCs, analyzed at 4 months after HSCT. The percentage of GFP+ HSCs (donor-derived HSCs from primary recipients) infused to the secondary recipients is shown; mice were pooled from 2 experiments. Data points and error bars represent mean ± SEM. Student’s t test (B, CD45-SAP), 1-way ANOVA (B, cKit-SAP), and repeated measures ANOVA (C) were used for statistical comparisons. *P < 0.05; ***P < 0.001; ****P < 0.0001.