Skip to main content
. Author manuscript; available in PMC: 2021 Dec 14.
Published in final edited form as: Methods. 2021 Mar 2;196:85–103. doi: 10.1016/j.ymeth.2021.02.020

Fig 7.

Fig 7.

Strategies for circRNA translation. (A) As an alternative to cap-mediated translation, translation of a synthetic circRNA can be initiated by inclusion of a viral IRES sequence or an m6A modification site upstream of the protein open reading frame (ORF). (B) Translation is further enhanced by the removal of putative stop-codons from a circRNA sequence with a length divisible by 3 to create an “infinite” ORF (right). This allows the ribosome to continually circumnavigate the same circRNA, producing a long, repeating peptide. Inclusion of a P2A site allows for self-cleavage of the resulting protein into homogenous monomeric units [123].