Figure 5.

HUVEC tube conditioned media caused breast spheroids to change their morphology. Preformed MCF10A or MCF10A-NeuN breast cell spheroids were incubated with conditioned media collected from HUVEC tube-like networks. In some conditioned media samples, EGF (20 ng/mL) was added as a positive control, or a neutralizing EGF antibody (0.1 μg/mL) was preincubated with conditioned media samples prior to adding media to breast spheroids. Samples were then fixed, labeled, and imaged by confocal microscopy after 24 h. (Integrin α6 = green and nuclei = blue). Scale bar: 50 μm.