Figure 6.

Cysteine depletion induces K562 cell ferroptosis after inhibiting the activity of TXNRD1. (a) Cell viability of K562 cells treated with auranofin or auranofin and Fer-1 and cultured in cysteine depletion condition was measured using the MTS kit. (b) TEM images showed the cell ultrastructure changes of K562 cells treated with auranofin under cysteine depletion condition (scale bar = 2 μm). (c, d) ROS and MDA levels of K562 cells treated with auranofin under cysteine depletion condition were measured. Auranofin: 1 μM. ^∗P < 0.05 vs. control group, ^∗∗P < 0.01 vs. control group, ^∗∗∗P < 0.001 vs. control group, ^#P < 0.05 vs. auranofin group, ^##P < 0.01 vs. auranofin group, and ^###P < 0.001 vs. auranofin group.