Table 8.
Box-Behnken factorial experimental design for amylase production by Streptomyces rochei HMM 13.
| Trials | Starch (X1) | K2HPO4 (X2) | pH (X3) | Temp. (°C) (X4) | A (U/ml) | P (mg/ml) | SA (U/mg) |
|---|---|---|---|---|---|---|---|
| 1 | 0 | 1 | 0 | 1 | 17.8227 | 10.8877 | 1.63696 |
| 2 | 1 | -1 | 0 | 0 | 14.1576 | 63.8529 | 0.22172 |
| 3 | 0 | 0 | 1 | -1 | 10.9595 | 13.2233 | 0.82880 |
| 4 | 1 | 0 | 0 | 1 | 13.0436 | 12.6484 | 1.03125 |
| 5 | 0 | 1 | -1 | 0 | 12.0375 | 45.4552 | 0.26482 |
| 6 | 0 | 0 | -1 | 1 | 16.6729 | 12.7562 | 1.30704 |
| 7 | 0 | 0 | 1 | 1 | 14.5888 | 71.8300 | 0.20310 |
| 8 | 1 | 0 | 1 | 0 | 14.5528 | 99.3906 | 0.14642 |
| 9 | -1 | 0 | 1 | 0 | 16.3854 | 37.3703 | 0.43846 |
| 10 | 0 | 0 | -1 | -1 | 15.7027 | 12.9358 | 1.21388 |
| 11 | 0 | 0 | 0 | 0 | 18.7210 | 71.0754 | 0.26339 |
| 12 | 0 | 1 | 0 | -1 | 15.5949 | 11.3548 | 1.37341 |
| 13 | -1 | 0 | 0 | 1 | 18.6492 | 41.0354 | 0.45446 |
| 14 | 0 | 1 | 1 | 0 | 15.3793 | 68.8835 | 0.22326 |
| 15 | 1 | 1 | 0 | 0 | 19.3319 | 8.55205 | 2.26050 |
| 16 | 0 | -1 | 0 | -1 | 13.9779 | 13.7982 | 1.01302 |
| 17 | 1 | 0 | 0 | -1 | 14.3372 | 18.1461 | 0.79009 |
| 18 | -1 | 0 | -1 | 0 | 13.0077 | 12.7562 | 1.01971 |
| 19 | -1 | -1 | 0 | 0 | 15.1277 | 24.8297 | 0.60926 |
| 20 | -1 | 0 | 0 | -1 | 13.4389 | 14.1935 | 0.94683 |
| 21 | 0 | -1 | -1 | 0 | 16.4932 | 29.3572 | 0.56181 |
| 22 | 0 | -1 | 1 | 0 | 15.5949 | 71.2910 | 0.21875 |
| 23 | 1 | 0 | -1 | 0 | 13.5108 | 44.7365 | 0.30200 |
| 24 | 0 | 0 | 0 | 0 | 12.4328 | 68.0930 | 0.18258 |
| 25 | 0 | -1 | 0 | 1 | 11.5704 | 25.4405 | 0.4548 |
| 26 | -1 | 1 | 0 | 0 | 14.1216 | 50.7014 | 0.2785 |
| 27 | 0 | 0 | 0 | 0 | 11.3189 | 62.1640 | 0.1820 |