Table 9.
Progression of purification fold for amylase production using Streptomyces rochei HMM 13.
| A (U/ml) | P (mg/ml) | SA (U/mg) | Ap (mm) | Protein recovery | P. fold | Yield% | |
|---|---|---|---|---|---|---|---|
| Basal medium | 0.227 | 0.407 | 56 | 24 | 1 | 100 | |
| Plackett-Burman | 30.001 | 10.82 | 277 | 33 | 2658 | 3.97 | 64.47 |
| Box-Behnken | 64.895 | 8.803 | 737 | 45 | 2162 | 12.22 | 24.23 |
| Ammonium sulfate (90%) | 67.91 | 7.22 | 940 | — | 1773 | 15.58 | 18.9 |
| Sephadex G-50 | |||||||
| First peak | 22.442 | 2.305 | 973 | 566.34 | 16.12 | 19.56 | |
| Second peak | 14.364 | 1.261 | 1138 | 309.8 | 18.85 | 22.87 | |
| Third peak | 14.966 | 1.68 | 890 | 412.78 | 14.74 | 17.88 |
Note: A (U/ml): activity; P (mg/ml): protein; SA (U/mg): specific activity; Ap (mm): agar diffusion plate; P. fold: purification fold.