Fig. 2.

MPO-dependent chlorolipid production in PMA-activated neutrophils. 2 x 10⁶/ml neutrophils were treated with (red) or without 200 nM PMA (black) for 30 min at 37 °C. Neutrophils were also treated with 10 mM 3-amino-1,2,4-triazole for 5 min prior to treatments with 200 nM PMA (green). 2-ClFALD (A), 2-ClFA (B) and LPE (C) molecular species were measured as described in “Materials and Methods”. ****p<0.0001 for comparisons between neutrophils treated with PMA to controls; **, ***, and ****p<0.01, 0.001, and 0.0001, respectively for comparisons between PMA treated and PMA treated with 3-amino-1,2,4-triazole pretreatment. Statistics were performed using ANOVA with Tukey multiple comparison test, error bars for ± SD, Data represents n=3 for A & B, and n=4 for C. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)