Fig. 5.

Immune group clusters are not differentiated by viral antigen burden.

(A) Patient sera matched to each liver biopsy was analyzed for viral biomarkers and ALT levels. The viral biomarkers HBV DNA, HBV RNA, HBcrAg, HBsAg and HBeAg were analyzed from each patient and timepoint and compared between immune groups. P values were calculated using unpaired t tests. (B) mIF channels for HBcAg, HBsAg, CD20 and CD3 were overlaid to visualize intrahepatic immune signatures in relation to viral antigens. Representative images from 4 individual samples representing immune high and immune low signatures at baseline with corresponding high or low viral antigen burdens are shown. HBcAg (red), HBsAg (green), CD3/CD20 merged (white) and DAPI (blue). IHC, immunohistochemistry; mIF, multiplex immunofluorescence.