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. 2021 Dec 1;12:732530. doi: 10.3389/fimmu.2021.732530

Figure 8.

Figure 8

Inhibition of SIRPα-γ/CD47 interactions alters memory cells in the spleen of humanized mice. At Day 14, splenocytes were analyzed by FACS. (A) Human chimerism is shown; data were analyzed with a Mann-Whitney test. (B) CD4/CD8 ratio is shown regarding the treatment; data were analyzed with a Mann-Whitney test. (C) Relative number of human CD4 and CD8 in the splenocytes of recipient mice at Day 14. Data were analyzed with a one-way ANOVA test followed by Kruskal-Wallis. (D) Percentage of human CD4 and (E) CD8 subpopulations is presented based on FACS analysis with the following patterns CD45RA−CD27− effector memory cells (EM), CD45RA−CD27+ central memory cells (CM), CD45RA+CD27+-naïve cells, and CD4−CD45RA+CD27− for TEMRA CD8 cells. (F) Relative number of each subpopulation of CD4+ and (G) CD8+ T cells are presented. n = 1 experiment with PBMC from one HV. Data were analyzed with a one-way ANOVA test followed by Kruskal-Wallis. * p ≤ 0.05; ** p ≤ 0.01; ns, not significant.