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. 2021 Oct 18;49(12):3621–3635. doi: 10.1007/s10439-021-02872-2

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Electrospun scaffold characterization, functionalization and EV attachment. (a) Schematic diagram of electrospinning and functionalization process. (b) SEM image of 8 mm electrospun PCL scaffold (inset: macro image of scaffold). (c–f) SEM imaging of scaffold fibre morphology of collagen functionalised and collagen-EV functionalised scaffolds respectively. (g and h) Confocal imaging of collagen-functionalized scaffold after incubation with a PKH-26 dye control or PKH-26 labelled EVs respectively.