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. Author manuscript; available in PMC: 2023 Jan 1.
Published in final edited form as: Bone. 2021 Nov 13;154:116257. doi: 10.1016/j.bone.2021.116257

Fig. 7. NAD(P)H FLIM of osteoblasts within healing defect or nearby native bone of Col (I) 2.3GFP mice.

Fig. 7.

MPLSM of a 1 mm defect performed 18 days after surgery (A). Representative images of native bone illustrate bone/SHG (white), osteoblasts (green), and vasculature (red) with highlighted region where NAD(P)H FLIM was performed on individual cells (A1–4) [scale bar = 10 μm]. Corresponding phasor plot of the region shows a distribution of points (A5) where selected regions from the phasor demonstrate separable species among collagen (highlighted orange) and GFP+ osteoblasts (highlighted green) (A6). Calculated NAD(P)H τM of GFP+ osteoblasts within the healing defect as well as the nearby uninjured native bone (B) as well as pO2 within measured regions (C). [N≈37 cells for NAD(P)H τM measurements and N≈9 pO2 point measurements in each of 4 mice; ***, p<0.001; Student’s t-Test]