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. Author manuscript; available in PMC: 2023 Feb 1.
Published in final edited form as: Toxicol In Vitro. 2021 Oct 5;78:105252. doi: 10.1016/j.tiv.2021.105252

Figure 3.

Figure 3.

Gel electrophoresis image of plasmid DNA treated with NPCuO (500 μM), H2O2 (50 μM), and EGC (0.5 – 800 μM) at pH 7 (MOPS buffer) for 150 min. Lane 0: 1 kb molecular weight ladder (with bands labeled in Figure S10); lane 1: plasmid (p); lane 2: p + H2O2 (50 μM); lane 3: p + EGC (800 μM); lane 4: p + NPCuO (500 μM) + H2O2 (50 μM); lanes 5–15: p + NPCuO (500 μM) + H2O2 (50 μM) + increasing concentrations of EGC (0.5, 1, 2, 5, 10, 25, 50, 100, 200, 400, and 800 μM, respectively). Damaged (nicked) plasmid DNA is in the top band, undamaged (supercoiled) DNA is in the bottom band, and the diffuse bands at lower molecular weights indicate significant DNA damage with many strand breaks per plasmid.