FIG. 3.

Histochemical analysis of ALP knockout mouse muscle. (A) Cryosections of gastrocnemius muscle from ALP^+/+ and ALP^−/− mice were analyzed with hematoxylin and eosin (H&E) staining. Sections from knockout mouse tissue resembled the wild-types and showed regularly sized and shaped myofibers and predominantly peripherally localized nuclei. (B) Immunofluorescent staining shows that ALP staining at Z lines is abolished in muscle sections from an ALP^−/− mouse. (C) The localization of α-actinin at skeletal muscle Z lines remains normal in an ALP^−/− mouse.