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. 2021 Dec 14;12(12):1158. doi: 10.1038/s41419-021-04451-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A The knockdown efficiency of ITGB1 was determined by western blot in RBE and QBC939 cell lines. B Western blot analysis of the efficiency of ITGB1 knockdown in ALDH3B2-overexpressing RBE (left) and QBC939 (right) cell lines. C CCK-8 assays were performed to identify the alteration in cell proliferation ability after ITGB1 knockdown in ALDH3B2-overexpressing RBE (upper) and QBC939 (lower) cells. Knockdown of ITGB1 reversed the promoting effect of ALDH3B2 overexpression on the proliferation ability of RBE and QBC939 cells. D Transwell assays without matrigel were performed to evaluate the migration ability of RBE and QBC939 cells after ITGB1 knockdown in ALDH3B2-overexpressing RBE (upper) and QBC939 (lower) cells. Knockdown of ITGB1 reversed the promoting effect of ALDH3B2 overexpression on the migration ability of RBE and QBC939 cells. E Transwell assays with matrigel were performed to evaluate the invasion ability of RBE and QBC939 cells after ITGB1 knockdown in ALDH3B2-overexpressing RBE (upper) and QBC939 (lower) cells. Knockdown of ITGB1 reversed the promoting effect of ALDH3B2 overexpression on the invasion ability of RBE and QBC939 cells. F EMT biomarkers, including E-cadherin, N-cadherin, and snail, were detected by western blot in ALDH3B2-overexpressing RBE cells (left) and QBC939 cells (right) with or without ITGB1 knockdown. EMT-promoting effect of ALDH3B2 overexpression in RBE cells and QBC939 cells was reversed by knocking down the expression of ITGB1. G Western blot analysis of the expression of ITGB1 and the phosphorylation level of its downstream signaling pathway in ALDH3B2-overexpressing RBE cells (left) and QBC939 cells (right) with or without ITGB1 knockdown. Knocking down the expression of ITGB1 partially reversed the effect of ALDH3B2 overexpression.