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. 2021 Dec 14;12:7270. doi: 10.1038/s41467-021-27588-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Modification ratios at the peptide level. n = 2 independent experiments were repeated, two technical replicates (injections) were conducted for each individual sample. Data are presented as mean values of two biological repeats and dots represent the two corresponding biologically repeats. b Heat map (shaded in red) of the modification extents at the peptide level. c Extracted ion chromatogram of modified and unmodified representative peptide 232–251. d CID product-ion (MS²) spectra of modified and unmodified peptide 232–251. Source data are provided as a Source Data file.