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. 2021 Dec 14;12:7270. doi: 10.1038/s41467-021-27588-8

Fig. 4. NanoPOMP helps to reveal conformational change and ligands binding.

Fig. 4

a Modification extents at the residue level for three binding states of hGLUT1 (grey-hGlut1 + Glucose; blue-hGlut1 + Cyb; orange-hGlut1 + Mal). n = 2 independent experiments were repeated, two technical replicates (injections) were conducted for each individual sample. Data are presented as mean values of two biological repeats and dots represent the two corresponding biologically repeats. Source data are provided as a Source Data file. b, c Residues interacting with CyB and Mal as identified by NanoPOMP. d Residues reporting conformational change (indicated by arrows) from the Mal-binding state to CyB-binding state. e Residues reporting conformational changes from unbound state to CyB-/Mal-binding state.