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. 2021 Dec 14;7:390. doi: 10.1038/s41420-021-00779-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A SYT8 protein expression levels in BxPC-3 and PANC-1 cells 48 h post-transfection of non-targeting scrambled siRNA (NCsiSYT8) or siRNA SYT8 (siSYT8); the SYT8 overexpression vector or the respective control vector (NCSYT8) was analyzed by western blotting. B Cell viability in BxPC-3 and Panc-1 cell lines post-transfection using the CCK-8 assay, with the absorbance measured at 450 nm. C, D Quantification and representative images from the colony-formation assay of cell viability in BxPC-3 and PANC-1 cell lines. E Cell apoptosis. Representative dot plots of SYT8-knockdown BxPC-3 cells and SYT8-overexpressing PANC-1 cells stained for Annexin-V/PI and measured by flow cytometry. F In vivo tumor growth assay. BALB/c nude mice were injected with BxPC-3 or PANC-1 cells expressing the respective siRNA or overexpression vector and monitored every week for up to 6 weeks. Tumors were then harvested to measure the volume. n = 6 per group. Data are presented as mean ± SD with at least three independent experiments. *P < 0.05.