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. 2021 Dec 14;11:24005. doi: 10.1038/s41598-021-03520-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Aging-related spontaneous NETosis. The proportion of spontaneous NETs and dsDNA released by neutrophils was evaluated by fluorescence microscopy (a) and PicoGreen staining (b) at 1, 6, 12, and 24 h. (a) Samples were stained with antibodies against citrullinated histone H3 (citH3, red) and myeloperoxidase (MPO, green) and DNA was counterstained with DAPI (blue). Original magnification, 40× ; scale bar, 50 μm. (b) DNA release was compared between different groups, and the data are represented as the median with range from six independent experiments, **P < 0.01.