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. 2021 Dec 14;11:24005. doi: 10.1038/s41598-021-03520-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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RNA-seq analysis in spontaneous NETosis. Neutrophils from three healthy control children were isolated and divided into two groups, which were further divided into 1 and 6 h groups. Total RNA of cells was harvested at 1 h (control group) and 6 h (spontaneous NETosis group) and RNA-seq data was analyzed as described in the Methods. (a) Volcano map of differentially expressed genes between aging neutrophils and controls. Values are presented as log2. Up- and down-regulated genes are colored in orange and blue, respectively. Autophagy-up and -down related genes are colored in red and green, respectively. Hierarchical clustering with heat map showing the RNA levels of differentially expressed (b) genes associated with autophagy and (c) genes in aging neutrophils and controls. Genes are ranked based on their fold-change. (d, e) Gene set enrichment scatter plot and overlapping gene set network plot showed differentially expressed genes enriched in autophagy pathways.