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. 2021 Dec 14;12:7257. doi: 10.1038/s41467-021-27596-8

Fig. 2. Cryo-EM application of HFBI film using human apoferritin as a test sample.

Fig. 2

a Cryo-EM montage micrograph (scale bar, 200 µm) of the HFBI film-covered ANTA foil grid with vitrified human apoferritin. b Cryo-EM montage micrograph (scale bar, 5 µm) of one square of the grid. The holes with good ice thickness and relatively low contamination for data collection are labelled in purple. c Representative cryo-EM micrograph of human apoferritin particles. Scale bar, 20 nm. d Power spectrum of the high magnification cryo-EM micrograph in (c). The central region is magnified (inset) and shows the diffraction spots of the HFBI film. e Cryo-EM map of human apoferritin at 1.96 Å resolution, and a representative region is magnified (right) with the fitted atomic model. Scale bar, 2 Å. f Gold standard FSC curve showing an overall resolution of 1.96 Å. g Rosenthal–Henderson plot with an estimated B-factor of 87.8 Å.