Skip to main content
. 2021 Dec 14;11:23935. doi: 10.1038/s41598-021-03442-1

Figure 4.

Figure 4

Detachment and re-plating of differentiated SH-SY5Y cells. Terminally differentiated SH-SY5Y cells were re-plated using trypsin–EDTA (0.05%), GCDR or versene and assessed for viability and expression of neuronal markers 48 h after re-plating. (a) Microscopic images of calcein (live) and EthD-1 (dead) labelled cells. Images were acquired using an EVOS FL Auto microscope with a ×20 objective lens (scale bar = 100 µm). βIII-tubulin images were acquired using the GFP filter (excitation 482/25 nm; emission 524/24 nm; exposure 110 ms), while Hoechst nuclei images were acquired using the DAPI filter (excitation 357/44 nm; emission 447/60 nm; exposure 19 ms). (b) Fluorescence emission of calcein and EthD-1 was measured to evaluate viability after re-plating. A one-way ANOVA test and Tukey’s post-hoc analysis was performed to evaluate statistical significance where ****p < 0.0001, ***p < 0.001, **p < 0.01 and *p < 0.05.